￥448.00~4781.00

(Calcium Independent Sortase A)

Sortases belong to a class of transpeptidases that employ the thiol group of an active - site cysteine to modify proteins. They achieve this by recognizing and cleaving the C - terminal sorting signal LPXTG (where X represents any amino acid) between the threonine (T) and glycine (G) residues. Sortase 2A.9 is an engineered variant originating from the wild - type Sortase of Staphylococcus aureus, and it demonstrates significantly higher activity compared to the wild - type enzyme. It is employed for the site - specific labeling of antibodies or proteins featuring the LAXTG recognition sequence (where X stands for any amino acid). This enzyme facilitates the attachment of various tags, such as peptides, DNA, carbohydrates, or fluorophores containing a polyglycine sequence (Gly)ₙ (where n ≥ 3 glycine residues). Sortase 2A.9 is safeguarded by U.S. Patent No. 10,202,593. \nApplication: \nSortase 2A.9 can recognize antibodies or genetically engineered proteins encompassing the .
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货号
FH-TE004
品牌
FuHeng
规格
10μg
表达系统
Expressed in E. coli.
分子量
Approximately 16.7 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.
蛋白序列
QAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATREQLNRGVSFAKENQSLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRNVKPTAVEVLDEQKGKDKQLTLITCDDYNEETGVWETRKIFVATEVK
纯度
＞98% by SDS-PAGE or HPLC.
推荐使用条件
Reaction Buffer:
300 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl₂, pH 7.5
Stop Solution:
500 mM EGTA, pH 8.0
配方
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, with 5%Trehalose, 0.02% (v/v) Tween-20, pH 7.0.
外观
Sterile filtered White lyophilized (freeze-dried) powder.
复溶
Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled wate
稳定性和存储
For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ und
产品分类
工具酶
目录价
￥448.00
现价
￥448.00

产品名称 (Calcium Independent Sortase A)
货号 FH-TE004
品牌 FuHeng
规格 10μg
表达系统 Expressed in E. coli.
分子量 Approximately 16.7 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.
蛋白序列 QAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATREQLNRGVSFAKENQSLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRNVKPTAVEVLDEQKGKDKQLTLITCDDYNEETGVWETRKIFVATEVK
纯度 ＞98% by SDS-PAGE or HPLC.
推荐使用条件 Reaction Buffer: 300 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl₂, pH 7.5 Stop Solution: 500 mM EGTA, pH 8.0
配方 Lyophilized from a 0.2 μm filtered concentrated solution in PBS, with 5%Trehalose, 0.02% (v/v) Tween-20, pH 7.0.
外观 Sterile filtered White lyophilized (freeze-dried) powder.
复溶 Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled wate
稳定性和存储 For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ und
特别声明 富衡细胞库承诺尽**努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，细胞库资料不保证其准确性。
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